食品科学2012，Vol.33Issue(14)：181-183,3.
高效液相色谱法测定成熟龙眼各部位游离鞣花酸含量
Determination of Free Ellagic Acid in Different Parts of Mature Longan by HPLC
摘要
Abstract
In this study, the contents of ellagic acid （EA） in different parts of mature longan cultivars Shixia and Chuliang were determined by HPLC. The sample extraction was achieved through hot reflux in 30 mL of 80% methanol aqueous solution for 2 h. The chromatographic separation was achieved on an Agilent C18 column （250 mm× 4.6mm i.d., 5 μm） using a mobile phase composed of methanol, water and glacial acetic acid （50：49.7：0.3, V/V） at a flow rate of 1 mL/min. The injection volume, column temperature and detection wavelength were set at 10 μL, 35℃ and 254 nm, respectively. The linear range for EA was 1 -- 200μg/mL with a regression coefficient of 0.999. HPLC results showed that the content of EA in different parts oflongan cultivars Shixia and Chuliang decreased in the following order： stone fruit 〉 peel 〉 pulp. The EA levels in the peel and pulp measured in this study were much higher than those reported in other fruits.关键词
龙眼/鞣花酸/高效液相Key words
longan/ellagic acid/high performance liquid chromatography(HPLC)分类
农业科技引用本文复制引用
刘玉革,刘丽琴,刘胜辉,魏长宾..高效液相色谱法测定成熟龙眼各部位游离鞣花酸含量[J].食品科学,2012,33(14):181-183,3.基金项目
中央级公益科研院所基本科研业务费专项 （）
