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气相色谱法测定海豹油中EPA、DPA、DHA含量的方法学研究

杨宜婷 张晓燕 区海燕

食品科学2012,Vol.33Issue(14):223-227,5.
食品科学2012,Vol.33Issue(14):223-227,5.

气相色谱法测定海豹油中EPA、DPA、DHA含量的方法学研究

Determination of EPA, DPA and DHA in Seal Oil by Gas Chromatography

杨宜婷 1张晓燕 1区海燕1

作者信息

  • 1. 无限极(中国)有限公司,广东广州510665
  • 折叠

摘要

Abstract

Objective: To establish a method for determining co -3 polyunsaturated fatty acids including eicosapentaenoic acid (EPA), docosapentenoic acid (DPA) and docosahexaenoic acid (DHA) in seal oil. Methods: Fatty acids in seal oil were methyl esterified using a KOH-methanol solution. The applicability of the developed method was validated. Results: This method revealed an excellent liner relationship for methylated EPA, DPA and DHA in the concentration range of 10--2000μg/mL with recovery rate of 96.93%-- 103.38% and RSD less than 2%. Conclusion: This method is simple, rapid, accurate and suitable for the analysis of fatty acids in seal oil.

关键词

海豹油/EPA/DPA/DHA/含量测定

Key words

seal oil/eicosapentaenoic acid (EPA): docosapentenoic acid (DPA): docosahexaenoic acid (DHA):content determination

分类

轻工纺织

引用本文复制引用

杨宜婷,张晓燕,区海燕..气相色谱法测定海豹油中EPA、DPA、DHA含量的方法学研究[J].食品科学,2012,33(14):223-227,5.

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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