食品与发酵工业2012，Vol.38Issue(4)：11-14,4.
ITS序列在酵母批量分子鉴定中的适用性
Internal Transcribed Spacer （ITS） Sequence as Measure for Systemic Classification of Yeasts
摘要
Abstract
The internal transcribed spacer （ITS） region of nuclear DNA is one of the most used locus for yeast i- dentification with growing sequence data deposited in public-access databases. This study extensively evaluated the feasibility of ITS sequence analysis for systemic classification of yeast isolates. The ITS regions of 623 yeast strains de- posited in CICIM-CU were amplified by PCR and sequenced, the sequences were compared to reference data available at the GenBank database by using BLAST. Most of fungal isolates （93.3%） were assigned to species level successful- ly. 40 strains （6.4%） were only can be assigned to genus level. 2 strains cannot be identified based on ITS se- quences analysis. This study shown ITS sequences were sufficiently sensitive for identifying most of yeasts, it can be powerfully and ordinarily used as first-round barcoding gene to systemic identification of bulk yeast isolates.关键词
酵母/ITS序列/同源性比对/分子鉴定Key words
yeast/ITS sequence/homology analysis/molecular identification分类
农业科技引用本文复制引用
陈源源,陈浩,石贵阳,王正祥..ITS序列在酵母批量分子鉴定中的适用性[J].食品与发酵工业,2012,38(4):11-14,4.基金项目
国家自然资源科技平台（No.2005DKA21208）和教育部科技基础条件平台（No.505006）资助 （）
