食品与发酵工业2012，Vol.38Issue(4)：15-19,5.
荧光原位杂交（FISH）技术研究窖泥微生物群落
Study on the Microbial Community in Pit Mud by Fluorescence in situ Hybridization
摘要
Abstract
In order to investigate the diversity of microbial community in pit mud by fluorescence in situ hybrid- ization, detection of pure culture and samples were carried out. The influenced factors on FISH were studied based on five pure culture strains including Escheriehia coli, Bacillus subtilis, Lactobacillus planetarium, Acetobacter rancens, Clostridium acetobytylicum. Then the microbial community in pit mud was further investigated by the modified meth- od. The results showed that the quantitative characterization was influenced by growth phase. The detection rate was above 82% in logarithmic and stationary phases for E. coli and L. planetarium whereas was significantly less in death phase for L. planetarium. The detected fluorescentce signal of dead cells was rather weak than live cells. For E. coli added in pit mud samples, the resolution of FISH was improved significantly through removing humic acid by alumi- num sulfate, the recovery was 46. 89% of the direct microscopic somatic cell count. FISH could effectively character- ize the bacteria and archaea community. Detection of bacteria and archaea visualized quantitatively by FISH is benefi- cial to study microbial community in cellular level.关键词
荧光原位杂交/窖泥/真细菌/古菌Key words
FISH/pit mud/bacteria/archaea分类
农业科技引用本文复制引用
吴冬梅,何翠容,牛美灿,彭昱雯,郑佳,金扬,黄钧,周荣清..荧光原位杂交（FISH）技术研究窖泥微生物群落[J].食品与发酵工业,2012,38(4):15-19,5.基金项目
国家自然科学基金 （）
