食品与发酵工业2012，Vol.38Issue(4)：39-43,5.
纤维素酶水解能力的影响因素及纤维素结构变化研究
Study on the Factors Affecting Enzymatic Hydrolysis Capability and the Structural Changes of Cellulose
摘要
Abstract
Enzymatic hydrolysis of cellulose and straw was investigated under different hydrolysis conditions, such as reaction time, temperature and pH value. The structural change of the substrates during the process was also analyzed by scanning electron microscope （SEM）. Results indicate that, the efficiency of enzymatic hydrolysis signifi- cantly varied with different pH values, and the optimal pH value was found to be in the range of 4.0 N 5.0. Moreo- ver, enzymatic hydrolysis efficiency was sensitive to temperature and the optimal value of temperature was 40 ~C. Un- der this condition, the production rate of glucose by straw and cellulose reached 56.32% and 35.80% , respectively. Furthermore, hydrolysis process of both substrates was almost completed within 24 hours, and reached the highest effi- ciency after 48 hour. SEM results show that the substrate structure underwent obvious change during the first 6 hours.关键词
纤维素酶/水解/秸秆糖化Key words
cellulase/hydrolysis/straw saccharification分类
轻工纺织引用本文复制引用
林燕,张伟,华鑫怡,李进军,刘妍,马海元..纤维素酶水解能力的影响因素及纤维素结构变化研究[J].食品与发酵工业,2012,38(4):39-43,5.基金项目
国家科技重大专项.水体污染控制与治理 （）
上海市自然科学基金 （）
污染控制与资源化研究国家重点实验室开放课题 （）
