食品与发酵工业2012，Vol.38Issue(4)：94-98,5.
东方伊萨酵母胞外多糖的单糖组成及抗氧化活性分析
Study on the Monosaccharide Composition of Exopolysaccharides from Issatchenkia orientalis and Its Antioxidant Activity
摘要
Abstract
In this research we separated the exopolysaccharide from fermentation medium of Issatchenkia oriental by ethanol precipitation, and pure exopolysaccharide（EPS-I） was obtained by DEAE-cellulose52 chromatography after re- moving protein and dialyses. The structure of pure exopolysaccharide were determined by UV scanning. Identification of IR showed that the exopo- lysaccharide had polysaccharides specific function group such as --OH ,--CH ,--C -- O etc. but they were not proteoglycan. The hydrolyzate of pure exopolysaecharide was analyzed by Gas chromatography and the result indicated that the exopolysaccharide was mainly consisted of L-mannose, D-xylose and D-rhamnose, and their content were 3.62% , 11.47% and 84.91% , respectively. Preliminary research on the antioxidant activity in vitro of EPS-I was done. Total antioxidant capacity （TAC） , the scavenging rate of DPPH ~ , hydroxyl radical and superoxide anion were investigated in vitro. The results demonstrated that EPS-I had favorable antioxidant activity, at the concen- tration of 20mg/mL, the scavenging activities of DPPH ~ and O~ ~ radicals of EPS-I could reach to 80% above.关键词
东方伊萨酵母/胞外多糖/纯化/组成/抗氧化Key words
Issatchenkia orientalis/exopolysaccharide/purification/composition analysis/antioxidant activity分类
轻工纺织引用本文复制引用
包怡红,梁雪,秦蕾,李锐达,王文琼..东方伊萨酵母胞外多糖的单糖组成及抗氧化活性分析[J].食品与发酵工业,2012,38(4):94-98,5.基金项目
黑龙江省归国科学基金资助项目 （）
