食品与发酵工业2012，Vol.38Issue(7)：11-16,6.
利用絮凝及双水相萃取分离纯化发酵液中的R，R-2，3-丁二醇
Fiocculation and Aqueous Two-phase Extraction of R ,R-2,3-Butanediol from Fermentation Broth
摘要
Abstract
R,R-2,3-Butanediol （R,R-2,3-BD） can been produced through one-step fermentation of inulin ex- tract from Jerusalem artichoke tubers using Paenibacillus polymyxa Z J-9. The pretreatment of fermentation broth by chitosan flocculation was investigated. The results were as following： the optimum flocculation was obtained using 0.75 g/L of chitosan with 43.5 ku molecular weight, 0. 125 g/L of sodium alginate, pH 5.0 and stirring for 15 mi- nutes. The flocculation rate of cells and proteins reached 89.46% and 78.93% , respectively, and the retained ratio of R,R-2,3-butanediol reached 98.54%. Then, the different aqueous two-phase extraction systems were used to fur- ther investigate extraction of R,R-2,3-BD from the pretreated fermentation broth and the isopropanol/ammonium sul- phate system was chosen as optimum system. The optimum phase composition was 33% （w/w） of isopropanol and 30.0% （w/w） of ammonium sulphate, and the partition coefficient and recovery of R,R-2,3-BD reached 7.96 and 89.40% , respectively. Furthermore, the isopropanol/ammonium sulphate aqueous two-phase extraction system could be used to separate different concentration R,R-2,3-BD from the pretreated fermentation broth by chitosan floceula- tion.关键词
PaenibaciUus/polymyxa/ZJ-9，R，R-2，3-丁二醇，絮凝，壳聚糖，双水相萃取Key words
Paenibacillus polymyxa/2/3-butanediol/flocculation/chitosan/aqueous two-phase extraction分类
化学化工引用本文复制引用
高健,薛锋,李凤伟,徐虹..利用絮凝及双水相萃取分离纯化发酵液中的R，R-2，3-丁二醇[J].食品与发酵工业,2012,38(7):11-16,6.基金项目
江苏省自然科学基金计划项目 （）
江苏省教育厅高校科研成果产业化推进项目 （）
盐城工学院科研基金项目（XKY2009006）. （）
