食品与发酵工业2012，Vol.38Issue(7)：26-31,6.
高效降解褐藻胶新菌种的筛选、鉴定及产酶条件优化
Isolation, Identification, and Fermentation Optimization of a High Efficient Novel Alginate-degrading Strain
摘要
Abstract
A high efficient novel alginate-degrading microorganism was isolated from rotten seaweed using sodium alginate as the sole carbon source. Based on the morphological, physiological characteristics and 16S rDNA sequence, the strain was identified as Isoptericola halotolerans WX. The optimized fermentation medium was composed of sodium alginate 6.0 g/L, tryptone 5.0 g/L, yeast extract 2.5 g/L, NaCl 25.0 g/L, MgSO4 2 mmol/L, CaCl2 0.5 mmol/L, KH2PO4 1 mmol/L, FeSO4 0.2 mmol/L, and MnSO4 0.3 mmol/L. The optimal culture condition was that the strain was cultured in 250 mL shake flasks containing 20 mL medium with shaking speed of 180 r/min and initial pH 8.0 at 25 ℃ for 44 h. Besides, 2 mmol/L Mg2＋ and 0.2 mmol/L Fe2＋ had remarkable effect on enzyme activity. Under the optimized conditions, the activity of alginate lyase was increased significantly from 32 U/mL to 432 U/mL, around 13-fold compared with the state without optimization. Additionally, Isoptericola halotolerans WX was demonstrated to possess the activity of amylase, which confers its extensive potential applications.关键词
褐藻胶裂解酶/嗜盐白蚁菌/褐藻寡糖/筛选/产酶条件优化/淀粉酶Key words
alginate lyase/Isoptericola halotolerans/alginate oligosaccharides/isolation/fermentation optimization/amylase分类
轻工纺织引用本文复制引用
魏丹,窦文芳,李恒,李会,许正宏,史劲松..高效降解褐藻胶新菌种的筛选、鉴定及产酶条件优化[J].食品与发酵工业,2012,38(7):26-31,6.基金项目
十二五国家科技支撑计划项目（2012BAD33806）,中央高校基本科研业务费专项资金（JUSRP31101）,江苏省自然科学基金 （）
