食品与发酵工业2012，Vol.38Issue(7)：48-52,5.
酶解法增溶酵母（1→3）-β-D-葡聚糖的研究
An Enzymolysis Method for the Solubilization of Yeast（1→3）-β-D-glucan
摘要
Abstract
The process of preparation water-soluble yeast （1→3）-β-D-glucan with enzymolysis was studied in this paper. Single-factor test and orthogonal test were conducted to study the influences of various factors on the yield of soluble polysaccharide. The optimal reaction conditions ： enzyme concentration 0.15 U/mL, substrate concentration 0.5 g/100mL, reaction temperature 40℃ , pH 3.5 and reaction time 0.5 h. The yield of soluble polysaccharide by these conditions is 80.3%. In order to determine the triple helix structure and the molecular configuration of the samples, Congo Red test and FT-IR spectra were used. The soluble polysaccharide by enzymolysis can stimulate the im- mune system of mice. Not only can it extend the survival time of the mice which was challenged by E. coli, but also can enhance the survival of it.关键词
葡聚糖/酶解法/正交实验/红外光谱/生物活性Key words
glucan/enzymolysis/orthogonal test/FT-IR spectra/biological activity分类
轻工纺织引用本文复制引用
张学况,杜丽平,王超,杜雨轩,肖冬光..酶解法增溶酵母（1→3）-β-D-葡聚糖的研究[J].食品与发酵工业,2012,38(7):48-52,5.基金项目
天津科技大学科学研究基金项目 （）
