黏质沙雷氏菌胞外几丁质酶的纯化及特性OA北大核心CSCDCSTPCD
Purification and Characterization of Extracellular Chitinase from Serratia marcescens
黏质沙雷氏菌几丁质酶发酵上清液经硫酸铵沉淀、透析、DEAE一琼脂糖凝胶阴离子交换层析和苯基-琼脂糖凝胶疏水层析，得到电泳纯的几丁质酶和几丁质结合蛋白CBP21。该几丁质酶和CBP21分子质量分别约为58ku和21ku，CBP21对该几丁质酶水解几丁质增效明显。几丁质酶反应最适温度为50~C，最适pH约为6．5～7．0。该酶在55℃以下、pH4．5～8．0范围内稳定。酶的Km值为0．22mg／mL，k为1．26Ixmol／（min·mg）。金属离子…查看全部>>
An extracellular chitinase and a chitin-binding protein （CBP21） were isolated from the culture of Serratia marcescens and purified to electrophoretic homogeneity by ordinal procedures containing ammonium sulfate precipitation, DEAE-Sepharose and Phenyl-Sepharose chromatography. Their relative molecular masses were estimated to be respectively about 58kD and 21kD by SDS-PAGE. CBP21 had great synergistic effect with the chitinase on chitin hydrolysis. The opti…查看全部>>
施腾鑫;黄秀菁;刘嘉;贺淹才
福建福大百特科技发展有限公司酶高效表达国家工程实验室,福建福州350000福建福大百特科技发展有限公司酶高效表达国家工程实验室,福建福州350000华侨大学工业生物技术研究所,福建泉州362021华侨大学工业生物技术研究所,福建泉州362021
生物科学
黏质沙雷氏菌几丁质酶纯化特性
Serratia marcescenschitinasepurificationcharacterization
《食品与发酵工业》 2012 (7)
114-119,6
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