食品与机械2012，Vol.28Issue(4)：89-92,4.DOI:10.3969/j.issn.1003-5788.2012.04.024
UPLC-MS-MS法测定水产品中克百威及其代谢物
Determination of carbofuran and its metabolites in aquatic products by UPLC-MS-MS
摘要
Abstract
An ultra performance liquid chromatography tandem mass spectrometric method (UPLC- MS/MS) has been developed for the simultaneous determination of carbofuran and its main metabolites (3-hydroxycarbofuran , 3-ketocarbofuran and carbofuran phenol) in aquatic products. The carbofuran and its main metabolites were extracted with acetonitrile , cleaned up with HLB solid phase extraction cartridge (SPE) . The UPLC analyses were performed on an ACQUITY HSS T3 column with gradient evaluation, combined with electrospray ionization in positive mode (ESI+)and multiple reaction monitoring(MRM)mode. The linear range was 0. 08-100 ng/mL, and the correlation coefficients were 0. 998 3~0. 999 7. The average recoveries of carbofuran and its main metabolites in fish and shrimp (0. 25 to 2. 5 μg/kg) ranged from 87. 9% to 96. 1 % and the relative standard deviations were between 1.4% and 9. 5 %. The detection limit of carbofuran, 3-hydroxy carbofuran, 3-keto carbofuran and carbofuran phenol was 0. 25 μg/kg . The method is easy, rapid, sensitive and suitable for the determination of carbofuran and its main metabolites in aquatic products.关键词
超高效液相色谱-串联质谱/水产品/克百威/3-羟基克百威/3-酮基克百威/呋喃酚Key words
Ultra performance liquid chromatography-tandem nass speetrometry( UPLC - MS - MS) / aquatic products/ carbofuran/ 3-hydroxycarbofuran/ 3-ketocarbofuran/ carbofuran phenol引用本文复制引用
何亚斌..UPLC-MS-MS法测定水产品中克百威及其代谢物[J].食品与机械,2012,28(4):89-92,4.基金项目
上海市技术型贸易措施应对专项(编号:10TBT004) （编号:10TBT004）
