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人参锈腐病拮抗细菌HNO1发酵条件优化

傅俊范 李玉娥 周如军 李大刚 苏丹

沈阳农业大学学报2012,Vol.43Issue(1):12-17,6.
沈阳农业大学学报2012,Vol.43Issue(1):12-17,6.

人参锈腐病拮抗细菌HNO1发酵条件优化

Optimal Fermentation Condition of Antagonistic bacteria Strain HNO1 Against Ginseng Rust Root Rot

傅俊范 1李玉娥 1周如军 1李大刚 1苏丹1

作者信息

  • 1. 沈阳农业大学植物保护学院,沈阳110161
  • 折叠

摘要

Abstract

The culture conditions and the compositions of cultural medium of Antagonistic bacteria strain HN01 against ginseng rust root rot were studied using the single factor and orthogonal design method. The result showed that the optimal fermentation conditions were 20% medium volume, 10% inoculation volume, shaking speed 150romin-l,pH 7, 30℃. The produce fungal fermentation medium was composed of 15.0g·L-1, beef extract 8g·L-1, NaCl 5g·L-1, (NH4)SO4 lmg·L-1 and K2HPO4 4g·L-1. Antifungal fermentation medium was composed sucrose l0g·L-1, beef extract 8g·L-1, NaCl 3g·L-1, (NH4)2SO4 2mg·L-1, K2HPO4 4g·L-1.

关键词

人参锈腐病/多粘芽孢杆菌/发酵条件/发酵培养基/正交试验

Key words

ginseng rust root rot/Paenibaciilus polymyxai fermentation condition/fermentation medium/orthogonal design

分类

农业科技

引用本文复制引用

傅俊范,李玉娥,周如军,李大刚,苏丹..人参锈腐病拮抗细菌HNO1发酵条件优化[J].沈阳农业大学学报,2012,43(1):12-17,6.

基金项目

辽宁省科技攻关项目(2007207002) (2007207002)

公益性行业科研专项经费项目(201107010-03) (201107010-03)

沈阳农业大学学报

OA北大核心CSCDCSTPCD

1000-1700

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