沈阳农业大学学报2012，Vol.43Issue(1)：48-52,5.
文冠果成熟胚离体培养及细胞学研究
In Vitro Culture and Cytological Studies of Mature Embryos of Xanthoceras sorbifolia
摘要
Abstract
In order to provide the scientific basis for the rapid propagation of Xanthoceras sorbifolia., the mature embryo of was taken as explants to investigate initial culture, subculture and rooting culture. It was found that the suitable media for adventitious buds induction in initial culture was MS+2.0mg ·L~16—BA+0.1mg ·cL-1NAA+30g·L-1sucrose+6g ·L-1agar,and the differentiation rate was 94.7% ;the suitable media for adventitious buds height growth in subculture was MS+0.5mg ?L-16-BA + 0.5mg·L-1NAA+30g·L-1sucrose+6g·L-1agar, then 50d after culture, the average seedling height (0.05cm) was 11.67 buds;the suitable media for rooting culture was WPM + 1.0mg·L-1IBA +30g·L-1sucrose+6g·L-1agar,and the rooting rate was 70.30%, the average root number was 3.57. The paraffin section showed the process of callus induction, differentiation of callus and adventitious buds development.关键词
组织培养/文冠果/成熟胚/不定芽/石蜡切片Key words
tissue culture/Xanthoceras sorbifolia/mature embryo/adventitious buds/paraffin section分类
农业科技引用本文复制引用
德永军,王一,叶冬梅,林涛,吕涛,吴婧..文冠果成熟胚离体培养及细胞学研究[J].沈阳农业大学学报,2012,43(1):48-52,5.基金项目
内蒙古自治区技术创新引导奖励资金重大科技项目(20091806) （20091806）
