沈阳农业大学学报2012，Vol.43Issue(1)：53-57,5.
培养基和土壤中木霉菌与绿僵菌微生态关系
Microecological Relationship Between Metarizium and Trichoderma in Artificial Medium and Soil
摘要
Abstract
The relationship between Trichoderma and Metarhizium cultured on the plate and in soil were studied respectively. The growth of Trichoderma and Metarhizium and the interacting effect between the two strains were observed according to the morphological colony confrontation on plate and re-isolation test from soil when the strains of Trichoderma and Metarhizium were inoculated at different proportion simultaneously. The similar observation was conducted when Metarhizium was inoculated and cultured first followed by inoculating Trichoderma at different intervals. The results showed that when Trichoderma and Metarhizium were inoculated simultaneously at the ratio of 1:100, the average growth distance of Trichoderma was as 6 times as that of Metarhizium on the plate. Metarhizium hardly isolated from Trichoderma after culturing for 4 days in soil. Therefore, Trichoderma inhibited to Metarhizium growth significantly, and it was hard for Metarhizium to grow due to the dominant growth of Trichoderma. When Metarhizium was inoculated and cultured for 7 days before inoculating Trichoderma, the growth distance of Metarhizium was lmm lower than that of Trichoderma, and the spores quantity of Metarhizium was higher than that of Trichoderma after the two strains were in a stable growth. The growth of Trichoderma turned to be weak and the growth rate of Metarhizium increased, consequently the growth of the two strains tended to be balanced, and it could achieve a certain level of co-existence. The thorough research need to be done further more in terms of the complex microbial ecological relationships between Trichoderma and Metarhizium in soil, in order to obtain the key techniques for avoiding the direct ecologic competition of the two strains and to realize the application of the two bio-control agents together synergistically.关键词
木霉菌/绿僵菌/协同作用Key words
Trichoderma/Metarhizium/synergistic effect分类
农业科技引用本文复制引用
衣思瑶,吕淑霞,陈捷,宋磊,黄磊,李雅乾..培养基和土壤中木霉菌与绿僵菌微生态关系[J].沈阳农业大学学报,2012,43(1):53-57,5.基金项目
农业部“948”项目(2011-G4) （2011-G4）
