沈阳农业大学学报2012，Vol.43Issue(2)：143-147,5.
苦参提取物抑制番茄灰霉病菌活性的研究
Studies on Antibacterial Activities of Sophora flavescens Extracts on Tomato Botrytis cinerea
摘要
Abstract
In this paper, the effect of Sophora flavescens extracts on Botrytis cinerea Pers were studied with mycelium growth rate method and suspending method. The results showed that all the concentrations (l,5,10,20g ?L-1) of Sophora flavescens restrained the mycelial growth of Botrytis cinerea Pers, and the stimulative rates were enhanced with the increased concentration. As the concentration of flavonoids was 20g·L-1, the max inhibition ratio of mycelial growth achieve 92.14% 4 days after inoculation. Sophora flavescens extracts strong restrained the spore germination of Botrytis cinerea Pers, with the concentration increasing of Sophora flavescens extracts, inhibitory effects on the spore germination promoted. In the concentration of 20g ? L"1, inhibition rate of spore germination was 97.46%, and all significant difference from that of the check test. The results of different temperatures, pH value, storage time on Sophora flavescens show that the antibacterial effect of Sophora flavescens extracts at -20℃ and l00℃ treatments were obvious. With the temperature increasing of Sophora flavescens extracts, the antibacterial effect declined obviously. After l00℃ treatment, the inhibition ratio declined to 62.43%. In certain pH value range (pH=5 to 9) the antibacterial effect of Sophora flavescens extracts has not changed much. When pH = ll, the inhibition ratio declined to 71.07%. In 4t environment, The effect of inhibitory ratio of sophora flavescens extracts that store 5d and Od had no difference. The effect of inhibitory ratio decreased with the storage time and was remarkably decreased when the time exceeded 21 days.关键词
苦参/番茄灰霉菌/抑菌活性Key words
Sophora flavescens/ Botrytis cinerea Pers/ antibacterial activity分类
农业科技引用本文复制引用
周宝利,刘双双,靳晓冬,尚淼,李兴宝..苦参提取物抑制番茄灰霉病菌活性的研究[J].沈阳农业大学学报,2012,43(2):143-147,5.基金项目
沈阳市科技计划项目(F10-226-4-00) （F10-226-4-00）
