沈阳农业大学学报2012，Vol.43Issue(2)：168-172,5.
响应面法优化小链霉菌产抗生素发酵条件
Optimization of Fermentation Condition for Antibiotic Production by Streptomycete parvus Using Response Surface Methodology
摘要
Abstract
Purpose: Optimizing the fermentation condition for antibiotic production by bacterial canker of tomato bio-control strain Streptomycete parvus using Response Surface Methodology (RSM). Methods: Firstly, the three of the most important influence factors were obtained through Plackett-Burman design screening. Then the path of steepest ascent experiment was adopted to approach the optimal region of the fermentation condition. Lastly the optimal concentration and mutual effect of three factors were obtained by RSM. Results: The best fermentation condition is: fermentation temperature 30.97 CC, pH 6.91 and 101.08 mL of packing volume in 250 mL triangle bottle. Under the above fermentation conditions the antibacterial ability of Streptomyces parvus could be enhanced to 38 mm, increasing 31.03% than before. Conclusions: Statistical experimental design and analysis has been successfully used for optimization of the fermentation condition of strain Streptomyces parvus effectively.关键词
小链霉菌/抗生素/响应面/发酵条件/Design ExpertKey words
Streptomyces parvus/ antibiotic/ RSM/ fermentation condition/ Design Expert分类
农业科技引用本文复制引用
孙建龙,于基成,刘秋,王皓,闫建芳,赵柏霞,刘志恒..响应面法优化小链霉菌产抗生素发酵条件[J].沈阳农业大学学报,2012,43(2):168-172,5.基金项目
国家自然科学基金项目(31070005) （31070005）
中央高校基本科研业务费专项项目(DC10030102,DC10030106) （DC10030102,DC10030106）
