沈阳农业大学学报2012，Vol.43Issue(2)：189-194,6.
丽金龟亚科(Rutelinae)部分种类16S rRNA-ND1基因序列分析
Sequence Analysis of Mitochondrial 16S rRNA-ND1 Gene from the Partial Species in Rutelinae (Coleoptera: Scarabaeidae)
摘要
Abstract
The sequences of 16S ribosomal RNA (rrnL) with the adjacent regions NAD dehydrogenase subunit 1 (nadl) and tRNA leucine gene sequenced from 7 samples of Rutilinae (Coleoptera: Scarabaeidae) were compared with the homologus sequences for other 11 samples downloaded from GenBank. The sequence composition and genetic distances were analyzed and the molecular phylogenetic trees were reconstructed using DNAStar5.0 and MEGA3.1 software. The results showed and that there were 218 variable sites and 1S5 parsimony-informative sites in the alignment sequences spanned 810 bp sites. The species exhibited a 73.3% Arginine and Thymine bias. The genetic distances within 9 genera in the subfamily Rutelinae ranged from 0.054 to 0.152 and within species in the different genera from 0.021 to 0.064. The related relationship between Popillia quadrigullata and Popillia japonica was quite close. The phylogenetic trees reconstructed with the methods of neighbor-joining and minimum evolution using Protaetia brevitareis and Heteronychus lioderes as outgroups reveal that 16 species representing 9 genera were divided into two lineages. The genera Prodoretus, Lepadoretus and Adoretus were grouped into one lineage. The genera Blitopertka, Phyllopertha. Mimela, Anisoplia, Anomala and Popillia were grouped into another one. The phylogenetic analysis trees put 5 species of the genus Anomala onto a single lineage and also indicated that molecular analysis is consistent with the traditional taxonomy based on the morphological characteristics.关键词
丽金龟亚科/线粒体DNA/16S rRNA-ND1基因/序列比较Key words
Rutilinae/ mitochondrial DNA/16S rRNA-NDl gene/ sequence comparison分类
生物科学引用本文复制引用
郭晓华,张媛,刘广纯,张卓,王剑峰,方红..丽金龟亚科(Rutelinae)部分种类16S rRNA-ND1基因序列分析[J].沈阳农业大学学报,2012,43(2):189-194,6.基金项目
辽宁省科技厅农业攻关计划项目(2008214001) （2008214001）
辽宁省自然科学基金项目(201102157) （201102157）
