中国食品学报2012，Vol.12Issue(4)：195-201,7.
果汁DNA提取方法比较及柑橘属植物分子生物学检测技术的研究
Comparison on the Extraction Method of DNA and Study on the Biotechnological Detection of Orange Component in Juice
摘要
Abstract
Three methods, including CTAB Method, SDS-CTAB method and modified CTAB method, were used to extract DNA from both pure orange juice and juice beverage, then the quality and quantity of DNA were analyzed by speclrophotometer and PCR amplification; the orange-specific primers were designed according to the sequence of UDP-glucoxyltransferase protein gene. The results showed that modified CTAB method was more suitable for the juice DNA extraction than the other two methods; the orange component could be detecled by the orange-specific primers with a limit of detection of 10 pg/μL. The following detection of fruit juice samples showed that this method was suitable to identified the orange component in fruit juice and other relative food and can provide a technique base for research of fruit juice adulteration detection.关键词
果汁/橙/DNA提取/葡糖基转移酶基因/定性检测Key words
juice/ orange/ DNA extraction/ glucoxyltransferase/ qualitative detection引用本文复制引用
刘伟红,许文涛,商颖,程国灵,罗云波,黄昆仑..果汁DNA提取方法比较及柑橘属植物分子生物学检测技术的研究[J].中国食品学报,2012,12(4):195-201,7.基金项目
国家高技术研究发展计划(863)项目(2006AA10Z440) （863）
国家自然基金项目(No.30800770)和转基因生物重大专项(2008ZX08012-001)资助 （No.30800770）
