中国食品学报2012，Vol.12Issue(7)：216-221,6.
细菌纤维素高产菌株的鉴定及产物分析
Identification and Product Characteristics Analysis of Cellulose-Producing Strain
摘要
Abstract
Objective: To identify a gel-like membrane producing strain CIs51, investigate characteristics of the gel-like substance and provide feasible technology for industrial production. Methods: CIs51 was identified through morphology observation, physiological and biochemical characteristics and 16S rDNA sequence analysis. The gel-like membrane produced by CIs51 was analyzed through cellulase hydrolysis experiments, infrared (IR) spectrum and X-ray diffraction (XRD) analysis to determine its main components and characteristics. The yield of gel-like dry sheet and passage stability in citrus dregs solution media were also investigated to determine its industrial production prospect. Results: CIs51 was identified as Gluconacetobacter hasenii CIs51 through morphology observation, physiological and biochemical characteristics as well as results of 16S rDNA analysis. Cellulose accounted for a proportion of 94% in the gel membrane. Results of IR spectrum and XRD indicated that it was β structure glucoscan and I type bacterial cellulose, crystallinity index was 75%. CIs51 grew well and maintained passage stability in citrus dregs solution media with the yield of cellulose reaching to 10.17 g/L. Conclusions: Gluconacetobacter hasenii CIs51 was a new isolate with high yield of cellulose, which grew well in citrus dregs solution media and had potentiality of industrial production. ·关键词
细菌纤维素/鉴定/汉逊氏葡糖酸醋杆茵/柑橘果渣Key words
bacterial cellulose/ identification/ Gluconacetobacter hasenii/ citrus dregs引用本文复制引用
杨颖,贾静静,陆胜民,邢建荣,夏其乐,陈剑兵..细菌纤维素高产菌株的鉴定及产物分析[J].中国食品学报,2012,12(7):216-221,6.基金项目
浙江省重大科技专项国际合作项目(2008C14069)资助 （2008C14069）
