食品工业科技2012，Vol.33Issue(18)：139-142,4.
直投式青海弧菌冻干粉保护剂的筛选及优化
Screening and optimization of the freeze-dried protectants for the lyophilized Vibrio-qinghaiensis of direct vat set
摘要
Abstract
In order to obtain the best protectants for Vibrioqinghaiensis sp.-Q67 of direct vat set,the freeze-dried protectants was firstly screened by single factor and then optimized by response surface analysis.The study indicated that in many substances the skim milk,saccharose,soluble starch and sodium glutamate have outstanding protective effect.In response to the relative light unit（RLU） of the lyophilized Vibrioqinghaiensis sp.-Q67 after the regeneration for 30min,when taken skim milk 7.68%,sucrose 2%,soluble starch 0.25%,sodium glutamate 1% as the combined optimal formula,the relative light unit（RLU） could reach 1382.87%;when the volume of protectants was equal to the strain and the response time was 30min,the relative light unit（RLU） could achieve 1356%.关键词
直投式/青海弧菌Q67/冷冻干燥/保护剂/响应面分析Key words
Vibrio-qinghaiensis sp.-Q67/freeze-dried/protectants/response surface analysis分类
轻工纺织引用本文复制引用
黄盈盈,丁武..直投式青海弧菌冻干粉保护剂的筛选及优化[J].食品工业科技,2012,33(18):139-142,4.基金项目
发光菌快速检测陕西省自然基金项目 （）
