食品工业科技2012，Vol.33Issue(18)：186-189,4.
响应曲面法优化辅酶Q10产生菌发酵培养基
Optimization of fermentation medium of coenzyme Q10-producing strain using response surface methodology
摘要
Abstract
In order to increase the prodution of coenzyme Q10,coenzyme Q10-producing strain R-SL15 was used to obtain from the optimum fermentation medium.Plackett-Burman design and Box-Behnken response surface methodology（RSM） was used to optimize medium for coenzyme Q10 biosynthesis.According to the RSM analysis on the data of Box-Behnken design,optimal fermentation conditions for the highest coenzyme Q10 content in fermentation medium were set at glucose content of 18.90g/L,yeast extract content of 5.54g/L,（NH4）2SO4 content of 0.98g/L、KH2PO4 content of 0.95g/L,beef extract content of 6g/L,MgSO4·7H2O content of 0.75g/L,FeSO4·7H2O content of 100mg/L,NaCl content of 10g/L and distilled water of 1L.Under the optimal conditions,coenzyme Q10 content produced by R-SL15 could reach to 51.31mg/L,which was 99.34% higher than that of non-optimized medium which was 25.74mg/L.The regression model was highly significant（R2=0.9423） and showed good fitness to the experimental data,and it could be used to predict.关键词
辅酶Q10发酵/培养基优化/Plackett-Burman/响应曲面Key words
coenzyme Q10 fermentation/medium optimization/Plackett-Burman/response surface分类
轻工纺织引用本文复制引用
于子玲,袁亚宏,岳田利,高振鹏..响应曲面法优化辅酶Q10产生菌发酵培养基[J].食品工业科技,2012,33(18):186-189,4.基金项目
国家十二五科技支撑计划 （）
陕西省科技攻关 （）
西北农林科技大学基本科研业务青年项目 （）
