食品工业科技2012，Vol.33Issue(18)：202-204,3.
生长圈法应用于选育L-异亮氨酸菌株的条件优化
Optimization of L-isoleucine output measurement by growth circle method
摘要
Abstract
Growth circle method is a simple,convenient,economical and effective way to preliminarily screen bacteria with high L-isoleucine output.In this paper,some factors including the temperature of test culture medium when the directional bacteria were added to,the mass of directional bacteria,agar concentration of test plate and agar concentration of agar pieces which affected measurement by bacterial growth circle method were studied.The optimal method was given as follows：minimal medium was chosen for test plate and agar pieces,the upper and lower parts of test plate were 10mL mixed bacteria culture medium and 10mL minimal medium,separately and the concentration of agar was 1%,the concentration of agar in agar pieces was 1.5%,the incubation time,the amount optimal temperature of directional bacteria in mixed bacteria plate were 18h,4% and 55℃,respectively.关键词
L-异亮氨酸/生长圈法/钝齿棒杆菌/琼脂浓度Key words
L-isoleucine/growth circle method/Corynebacterium crenatum/agar concentration分类
轻工纺织引用本文复制引用
李宗伟,苏明杰,谈重芳,王雁萍,金庆生..生长圈法应用于选育L-异亮氨酸菌株的条件优化[J].食品工业科技,2012,33(18):202-204,3.基金项目
公益性行业（农业）科研专项经费 （）
