食品工业科技2012，Vol.33Issue(18)：217-220,4.
His-tag对亚油酸异构酶在大肠杆菌中可溶性表达的影响
His-tag effect on solubility of linoleic acid isomerase produced in E.coli
摘要
Abstract
In order to investigate the effect of 6×His-tag on the solubility expression of linoleic acid isomerase（PAI） from Propionibacterium acne in E.coli,recombinant E.coli strains over-expressing PAI,PAIH（PAI fused with C-terminal his-tag）,HPAI（PAI fused with N-terminal his-tag） were constructed,respectively.Recombinant enzymes were analyzed by SDS-PAGE and Western blot.Enzyme activities were verified by gas chromatogram.Under similar growth and inducible expression conditions,three recombinant E.coli strains successfully expressed recombinant proteins which had linoleic acid isomerase activity.The total recombinant protein production levels of three recombinant strains were similar,His-tag had a noticeable negative effect on protein solubility and C-terminal His-tag had a bigger negative effect on solubility of the recombinant protein than N-terminal His-tag.关键词
亚油酸异构酶/大肠杆菌/6×His-tag/可溶性Key words
linoleic acid isomerase/E.coli/6×His-tag/solubility分类
轻工纺织引用本文复制引用
张白曦,许正凯,汪昶沛,陈海琴,宋元达,陈卫,张灏..His-tag对亚油酸异构酶在大肠杆菌中可溶性表达的影响[J].食品工业科技,2012,33(18):217-220,4.基金项目
“十二五”国家863计划 （）
