食品工业科技2012，Vol.33Issue(19)：173-176,181,5.
耐热菌D-乳酸脱氢酶突变体的可视化建模和大肠杆菌中突变体对产苯乳酸的影响
Visual modeling mutants for D-lactate dehydrogenase form aquifex aeolicus and the effect of mutants on the production of phenyl lactate acid in E.coli
摘要
Abstract
Based on bioinformatics,the amino acid residues of conservative and activity center of D-lactate dehydrogenase(D-LDH),and the three-dimensional structure model of protein was analysised.The space conformation of visualization mutant had been constructed by homology modeling,the best mutant models were selected by the calculation of the distance and angle.The results showed that the 4 amino acid residues were relevant to the activity center in 20 conservative residues of D-LDH.After the models were compared,it was found that the big molecules substrates were obstructed by the benzyl of the residues of Phenylalanine(phe)or Tyrosine(try)on the 49 and 297 position.When F49A,Y279A,F49A and Y279A were mutated,the obstacles would disappear or weaken.The three mutants constructed were made a preliminary study,the results showed that IPTG or lactose could induce mutant to produce phenyl lactic acid in E.coli.The yield of phenyl lactic acid was higher in static culture than in vibration incubator,and the one of the F49A mutant(A.a.D-LDH-F49A strains)was higher than the one of the wild type(A.a.D-LDH strains)with lactose inducing.It would be a method of constructing gene engineering strain that visualization mutants models were compared and selected.关键词
突变体/可视化/苯乳酸/D-乳酸脱氢酶Key words
mutant/visual/PLA/D-LDH分类
轻工纺织引用本文复制引用
田晋红,刘琦,战丽萍,李小丽..耐热菌D-乳酸脱氢酶突变体的可视化建模和大肠杆菌中突变体对产苯乳酸的影响[J].食品工业科技,2012,33(19):173-176,181,5.基金项目
重庆市科技创新能力建设（CSTC,2009CB1010）资助项目 （）
