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黑木耳多糖-乳清蛋白复合物的制备及其抗原性的研究

齐晓彦 李春 张微 刘宁

食品工业科技2012,Vol.33Issue(19):232-235,4.
食品工业科技2012,Vol.33Issue(19):232-235,4.

黑木耳多糖-乳清蛋白复合物的制备及其抗原性的研究

Study on preparation and antigenicity activity of glycosylation products derived from whey protein and auricularia auricula polysaccharide

齐晓彦 1李春 2张微 3刘宁1

作者信息

  • 1. 黑龙江省乳品工业技术开发中心国家乳业工程技术研究中心,黑龙江哈尔滨150086 东北农业大学食品学院乳品科学教育部重点实验室,黑龙江哈尔滨150030
  • 2. 东北农业大学食品学院乳品科学教育部重点实验室,黑龙江哈尔滨150030
  • 3. 黑龙江省乳品工业技术开发中心国家乳业工程技术研究中心,黑龙江哈尔滨150086
  • 折叠

摘要

Abstract

Protein and polysaccharide covalent complex through Maillard could form protein-polysaccharide conjugations which showed excellent properties.And the conjugates of whey protein and auricularia auricula polysaccharides(AAP)were studied by means of the dry-heating glycosylated reaction.It was shown that after the whey protein and Auricularia auricula polysaccharides(AAP)of different mass ratio reacting for different time,the antigenicity of β-LG and α-LA were estimated by indirect competition ELISA.The results indicated that the glycolsylation of whey protein could reduce the antigenicity of β-LG and α-LA.The optimum reaction condition were WPI and AAP(1:1 weight ratio)for 24 hours could reduce the antigenicity of whey protein effectively,The antigenicity of bovine milk β-LG and α-LA was reduced by conjugation with AAP,about 75.7% and 25% respectively.

关键词

黑木耳多糖/多糖提取/乳清蛋白/美拉德反应/抗原性

Key words

auricularia auricula polysaccharides/polysaccharides extraction/whey protein/Maillard reaction/antigenicity

分类

轻工纺织

引用本文复制引用

齐晓彦,李春,张微,刘宁..黑木耳多糖-乳清蛋白复合物的制备及其抗原性的研究[J].食品工业科技,2012,33(19):232-235,4.

基金项目

“十二五”国家科技支撑计划 ()

黑龙江省教育厅 ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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