食品工业科技2012，Vol.33Issue(19)：294-296,300,4.
发酵液中γ-聚谷氨酸含量快速测定方法研究
Study on efficient determination method of poly-γ-glutamic acid in fermentation broth
摘要
Abstract
A turbid solution was formed through the reaction between γ-PGA and Cetyl Trimethyl Ammonium Bromide(CTAB)sodium hydroxide solution,the preparation of different concentrations of γ-PGA standard solution with CTAB solution to form a reaction system,and the formation to make a full wavelength scan,draw the standard curve of different wavelengths,and CTAB reagent concentration,reaction time and temperature on the reaction system were optimized.The results showed that CTAB turbidimetry best detection wavelength was 250nm,CTAB mass concentration of 5g/L,reaction temperature was room temperature,complexing 3min.The linear regression equation y=0.0335x-0.1519,R2=0.9995.The precision and recovery of this method were within 2.56% and 106.6%.The turbidimetric method has advantages of convenience,simplicity and good repeatability and can be used to detect the γ-PGA concentration in the fermentation broth.关键词
γ-聚谷氨酸/十六烷基三甲基溴化铵（CTAB）/发酵液/定量分析Key words
γ-PGA/CTAB/fermentation broth/quantitative analysis分类
轻工纺织引用本文复制引用
张庆庆,金鑫强,陈剑翔,赵爱兵,李慧静..发酵液中γ-聚谷氨酸含量快速测定方法研究[J].食品工业科技,2012,33(19):294-296,300,4.基金项目
安徽省高校自然科学基金资助项目 （）
