食品工业科技2012，Vol.33Issue(20)：64-65,69,3.
高效液相色谱-示差折光法测定甜菊糖中莱鲍迪苷A的含量
Separation and identification of rebaudioside A in stevia by high performance liquid chromatography-refractive index detector
钱凯 1宋明军 2李洁莉 1李红梅 2杨建涛 3尤继明2
作者信息
- 1. 江苏省产品质量监督检验研究院,江苏南京210007
- 2. 国家有机食品质量监督检验中心,江苏宝应225800
- 3. 河南农业大学林学院环境系,河南郑州450002
- 折叠
摘要
Abstract
A high performance liquid chromatography method for separation and determination of rebaudioside A(RA) in stevia was established. The analytical column was Waters NH2(250mm×4.6mm,5um),the mobile phase was acentonitrile-water(80:20,V/V),the samples were determined by an refractive index detector detector. The calibration curves had good linearity in the range of 0.1-1.0mg/mL for RA,the detection limit for RA was 0.03mg/mL,the average recoveries of RA were 98.37% (n=6,RSD=0.88%). The method was convenient, rapid and effective for qualitatively analysis of RA in stevia.关键词
甜菊糖/菜鲍迪苷A/高效液相色谱/示差折光检测Key words
stevioside/rebaudioside A/HPLC/refractive index detector分类
轻工纺织引用本文复制引用
钱凯,宋明军,李洁莉,李红梅,杨建涛,尤继明..高效液相色谱-示差折光法测定甜菊糖中莱鲍迪苷A的含量[J].食品工业科技,2012,33(20):64-65,69,3.
