食品工业科技2012，Vol.33Issue(20)：84-86,90,4.
盐离子浓度对氧自由基吸收能力（ORAC）检测准确性的影响
Effect of salt ion concentration on the accuracy of oxygen radical absorption capacity(ORAC) detection
摘要
Abstract
The oxygen radical absorbance capacity(ORAC) assay method has been employed extensively in the field of antioxidant and oxidative stress. The ignorance of the effect of salt ion in the sample on ORAC method resulted in the incorrect evaluation of the antioxidant capacity. The present study investigated the effect of salt on ORAC assay's accuracy in terms of adding different salt(including NH4Cl,CH3COONa,CH3COONH4 and NaCl) to Trolox,ORAC values increased due to the the salt ions in the sample,especially CH3COONa,which may be attributed to the fact that the change of ionization of fluorescein caused by salt ion lead to the alteration of its oxidation rate. Therefore,measuring error could be eliminated by addition of same concentration of salt ion in tested sample in Trolox for determination of standard curve.关键词
氧自由基吸收能力/盐离子/Trolox氧化/荧光氧化Key words
oxygen radical absorption capacity/salt ion/Trolox oxidation/fluorescence oxidation分类
轻工纺织引用本文复制引用
顾敏,赵谋明,任娇艳..盐离子浓度对氧自由基吸收能力（ORAC）检测准确性的影响[J].食品工业科技,2012,33(20):84-86,90,4.基金项目
基金项目：国家自然科学基金（31000759）：广东省重大科技专项资助项目 （）
广东省部产学研项目（2011A090200078）. （）
