食品工业科技2012，Vol.33Issue(20)：156-159,4.
基于洛美沙星免疫原的喹诺酮药物广谱特异性抗体制备的研究
Preparation of broad-specific antibody to fluoquinolone drugs based on Iomefloxacin immunogen
摘要
Abstract
Using Iomefloxacin as hapten,the immunogen was prepared with water soluble carbodiimide (EDC) method,and the coating agent was synthesized with active ester method. The polyclonal antibody was obtained successfully after immunization. Characterized by indirect competitive enzyme linked immunosorbent assay(icELISA) ,the antibody exhibited strong broad-specificity, 11 fluoroquinolone drugs could be recognized with a limit of detection lower than 10ng/mL,which could meet the requirement of related international organization. This study would benefit to the immunoassay development for the simultaneous detection of many fluoroquinolone drugs.关键词
洛美沙星/抗体/广谱特异性/间接竞争酶联免疫检测Key words
Iomefloxacin/antibody/broad-specificity/icELISA分类
轻工纺织引用本文复制引用
Uwamahoro Emmanuellat,吕书为,唐秋实,王弘,沈玉栋,徐振林,孙远明,杨金易,雷红涛..基于洛美沙星免疫原的喹诺酮药物广谱特异性抗体制备的研究[J].食品工业科技,2012,33(20):156-159,4.基金项目
基金项目：国家自然科学基金 （）
高等学校博士点基金优先发展领域项目 （）
广州市科技计划项目（12C12101663）. （）
