食品工业科技2012，Vol.33Issue(20)：226-230,5.
响应面法优化蜂胶黄酮提取工艺及其抗氧化活性研究
Optimization of extraction process of propolis flavonoids by response surface methodology and its antioxidant activity research
摘要
Abstract
Propolis flavonoids were extracted by ethanol,and the extraction process was optimized by response surface analysis. Qn the basis of one-factor-at-a-time experiment,optimal extraction conditions for maximizing popolis flavonoids were determined by using a 4-variable,3-1evel Box-Behnken experimental design combined with response surface analysis as follows ;the liquid-material ratio was 14:1 (mL. g-1),the extraction temperature was 72.6℃ ,the extraction time was 2.5h,the ethanol concentration was 80% (v/v), resulting in an propolis flavonoids yield of 33.82% ,which was basic agreement with the predicted value of 33.9324%. Thus,the optimal process was reliable. The antioxidant activity in vitro condition of propolis fiavonoids were evaluated by DPPH free radical scavenging assay and potassium ferricyanide reduction assay using Vc as the control. Propolis flavones were found to have powerful DPPH free radical scavenging capacity and reducing power.Thus, propolis flavonoids could be developed as excellent natural antioxidant resources.关键词
蜂胶/黄酮/响应面法/抗氧化活性Key words
propolis/flavone/response surface methodology (RSM)/antioxidant activity分类
轻工纺织引用本文复制引用
李富华,夏春燕,刘燕妮,明建..响应面法优化蜂胶黄酮提取工艺及其抗氧化活性研究[J].食品工业科技,2012,33(20):226-230,5.基金项目
基金项目：国家星火计划项目（2010GA811001）. （）
