食品工业科技2012，Vol.33Issue(21)：172-177,6.
产高活性木聚糖酶放线菌的筛选及其产酶条件的优化
Screening of high-yield xylanase-producing Actinomycete sp.and optimization of its fermentation conditions
摘要
Abstract
52 actinomycetes were isolated from the soil samples of different districts of China based on plate screening method,and 12 strains was obtained with enzyme activity over 100U/mL.Actinomycete F4712 with high xylanase-producing(the initial enzyme activity was 349.4U/mL)was selected for further research.The conditions including:carbon source, nitrogen source, initial pH value, rotation rate, temperature and cultivation time were investigated by single-factor experiment.Box-Behnken was utilized to the response surface analysis to determine the conditions further,which were insoluble corncob xylan 2.8%,a combination of beef peptone 1.0% and yeast extract 0.5%, pH 6.2,130r/min,45℃ and 6 days.The xylanase activity was 693.4U/mL under the optimal conditions, which was improved by 98.5% compared to the original activity.关键词
放线菌/木聚糖酶/筛选/优化Key words
actinomyces/xylanase/screening/optimization分类
轻工纺织引用本文复制引用
滕超,纪烨,李秀婷..产高活性木聚糖酶放线菌的筛选及其产酶条件的优化[J].食品工业科技,2012,33(21):172-177,6.基金项目
国家自然科学基金项目 （）
北京市属高等学校人才强教计划资助项目（PHR20110872）. （）
