食品工业科技2012，Vol.33Issue(21)：178-180,3.
岷山红三叶草异黄酮合酶基因的克隆及序列分析
Cloning and sequence analysis of isoflavones synthase gene from Trifofium Pratense Minshan
摘要
Abstract
For making good use of the specific biological function of isoflavone.A cDNA fragment from full length IFS gene was amplified by polymerase chain reaction from total RNA of callus Trifolium Pratense Minshan, and it was sequenced after cloned into pGEM-T Easy vectors.A cDNA fragment of 1575bp from full length IFS gene was got. The sequencing results indicated that the cloned fragment of IFS contained 1575 nucleotides, and shared a sequence homology of 99% with that from Genbank accession.关键词
岷山红三叶/异黄酮合酶/基因克隆/序列分析Key words
Trifolium Pratense Minshan/isoflavone synthase/gene cloning/sequence analysis分类
农业科技引用本文复制引用
胡焕焕,刘瑞,姬国杰,李卫东,丰慧根..岷山红三叶草异黄酮合酶基因的克隆及序列分析[J].食品工业科技,2012,33(21):178-180,3.基金项目
河南省科技厅重点支持项目 （）
河南省教育厅立项项目 （）
新乡医学院研究生科研创新支持项目（YJSCX201116Y）. （）
