食品科学2012，Vol.33Issue(15)：1-5,5.
溶菌酶协同高压CO_2对酸土脂环酸杆菌芽孢的致死效果
Effects of Various Treatment Conditions on Efficacy of High Pressure Carbon Dioxide in Coordination with Lysozyme for Inactivating Alicyclobacillus acidoterrestris Spores
摘要
Abstract
One-factor-at-a-time and orthogonal array design methods were used to optimize four treatment conditions including lysozyme dose,pressure,temperature and treatment time that influence the bactericidal activity(evaluated by death rate of spores) of lysozyme in coordination with high pressure carbon dioxide against Alicyclobacillus acidoterrestris spores.The results showed that lysozyme dose,pressure and temperature had a significant effect on the inactivation of Alicyclobacillus acidoterrestris spores(P 0.05).The death rate of Alicyclobacillus acidoterrestris spores reached 5.47 orders of magnitude under the optimized conditions: lysozyme dose 0.10 g/kg,pressure 25 MPa,temperature 55 ℃ and treatment time 60 min.The HPLC analysis and observation under scanning electron microscopy(SEM) of Alicyclobacillus acidoterrestris spores treated under these conditions indicated large amounts of 2,6-pyridine dicarboxylic acid(DPA) leakage and complete rupture of partial spores.In conclusion,lysozyme has a significantly synergistic effect on the inactivation of Alicyclobacillus acidoterrestris spores by high pressure carbon dioxide(P 0.05).关键词
高压CO2/溶菌酶/酸土脂环酸杆菌/芽孢致死率/2,6-吡啶二羧酸（DPA）/扫描电子显微镜镜（SEM）Key words
high pressure carbon dioxide/lysozyme/Alicyclobacillus acidoterrestris/death rate of spores/2/6pyridine dicarboxylic acid(DPA)/scanning electron microscopy(SEM)分类
轻工纺织引用本文复制引用
周先汉,吴克平,曾庆梅,邹旭鹏,林秀峰..溶菌酶协同高压CO_2对酸土脂环酸杆菌芽孢的致死效果[J].食品科学,2012,33(15):1-5,5.基金项目
国家自然科学基金项目（30871739 （）
30571304） （）
国家“863”计划项目 （）
安徽省教育厅重点科研项目 （）
