食品科学2012，Vol.33Issue(15)：122-126,5.
丰年虫水溶性蛋白的分离纯化及体外抗氧化性研究
Antioxidant Activity of Water-Soluble Protein Purified from Eubranchipus vernalis
摘要
Abstract
Objective: To isolate and purify water-soluble protein with the highest antioxidant activity from Eubranchipus vernalis and determine its molecular weight.Methods: Crude water-soluble protein from Eubranchipus vernalis was extracted and purified by ammonium sulfate precipitation,salting out,ion-exchange column chromatography,DEAE-fast-flow Sepharose anion-exchange chromatography and Sephadex G-100 column chromatography.The antioxidant activities of different protein fractions were compared and their molecular weights were determined by SDS-PAGE.Results: The prepared crude watersoluble protein was mainly composed of 4 fractions named as Peak-1,Peak-2,Peak-3 and Peak-4 in DEAE-Sepharose fast-flow column chromatography and Peak-2 revealed higher antioxidant activity than other 3 fractions.In addition,Peak-2 was mainly composed of 3 sub-fractions including Peak 2-1,Peak 2-2 and Peak 2-3 in Sephadex G-100 gel-permeation column chromatography.Peak 2-1 exhibited higher antioxidant activity than other 2 sub-fractions.Peak 2-1 was identified by SDS-PAGE to reveal a molecular weight of 82.47 kD.The antioxidant activity of Peak 2-1 was much higher than that of the crude protein extract.Conclusion: Peak 2-1 has obtained from Eubranchipus vernalis as the most antioxidant protein with a molecular weight of 82.47 kD.关键词
丰年虫/水溶性蛋白/分离纯化/抗氧化活性Key words
Eubranchipus vernalis/water-soluble protein/purification/antioxidant activity分类
轻工纺织引用本文复制引用
聂路,张建新,李文学,刘胐..丰年虫水溶性蛋白的分离纯化及体外抗氧化性研究[J].食品科学,2012,33(15):122-126,5.基金项目
国家大学生创新性实验计划项目 （）
