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桦褐孔菌子实体与菌丝体三萜化合物提取及活性比较研究

朱春玉 郑方亮 邵丽杰 冯华炜 郭立雄 刘宏生

食品科学2012,Vol.33Issue(15):161-165,5.
食品科学2012,Vol.33Issue(15):161-165,5.

桦褐孔菌子实体与菌丝体三萜化合物提取及活性比较研究

Extraction and Bioactivity of Triterpenoids from Fruit Bodies and Mycelia of Inonotus obliquus

朱春玉 1郑方亮 1邵丽杰 1冯华炜 1郭立雄 1刘宏生1

作者信息

  • 1. 辽宁大学生命科学院,辽宁省生物大分子计算模拟与信息处理工程研究中心,辽宁省动物资源与疫病防治重点实验室,辽宁沈阳110036
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摘要

Abstract

In order to explore the difference in medicinal value of cultured mycelia and fruit bodies of Inonotus obliquus,organic solvent cold soaking and ultrasonic reflux were adopted to extract triterpenoids from fruit bodies and mycelia of Inonotus obliquus.The triterpenoids extracted from both sources were determined to be the same or similar homologues by thin layer chromatography(TLC) and Fourier transform infrared(FT-IR) spectral analysis.Both triterpenoid extracts could inhibit the growth of cancer cells and bacteria as determined by tetrazolium salt assay(MTT) and cylinder plate method.The highest inhibitory rate of triterpenoids to human gastric cancer cell line MGC-803 reached up to 38.27%.Therefore cultured mycelia of Inonotus obliquus have the same medicinal value as fruit bodies.

关键词

桦褐孔菌/三萜化合物/抗肿瘤/抑菌活性

Key words

Inonotus obliquus/triterpenes/anti-tumor activity/anti-bacterial activity

分类

生物科学

引用本文复制引用

朱春玉,郑方亮,邵丽杰,冯华炜,郭立雄,刘宏生..桦褐孔菌子实体与菌丝体三萜化合物提取及活性比较研究[J].食品科学,2012,33(15):161-165,5.

基金项目

辽宁省教育厅重点实验室项目 ()

辽宁大学青年科研基金项目 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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