食品科学2012，Vol.33Issue(15)：206-209,4.
基因组改组快速提高谷氨酸棒杆菌L-鸟氨酸产量
Genome Shuffling for Rapid Improvement of L-Ornithine Production by Corynebacterium glutamicum
摘要
Abstract
Genome shuffling was applied to Corynebacterium glutamicum ATCC13032 to achieve rapid improvement of L-ornithine production.Six mutant strains with subtle improvements in L-ornithine production were obtained by treatment with UV light,nitrosoguanidine or ethyl methane sulfonate and subjected to recursive protoplast fusion.The effects of medium components on the protoplast regeneration rate were investigated.High-yield strains were screened using plates containing different concentrations of sulfaguanidine and NaF.After two rounds of genome shuffling,two shuffled strains producing L-ornithine at high level with good genetic stability were selected and named as F2-6 and F2-29.After 72h of shake-flask fermentation,the L-ornithine yield of strain F2-6 reached 2.99 g/L,which was 13.6 times higher than that of the original strain.关键词
基因组改组/谷氨酸棒杆菌/L-鸟氨酸/产量Key words
genome shuffling/Corynebacterium glutamicum/L-ornithine/production分类
生物科学引用本文复制引用
张媛媛,李家洲..基因组改组快速提高谷氨酸棒杆菌L-鸟氨酸产量[J].食品科学,2012,33(15):206-209,4.基金项目
国家自然科学基金面上项目 （）
