食品科学2012，Vol.33Issue(15)：257-261,5.
响应面法优化短短芽孢杆菌FM4B的发酵培养基
Medium Optimization for Antifungal Substance Production by Brevibacillus brevis FM4B Using Response Surface Methodology
摘要
Abstract
Plackett-Burman design was used to evaluate the effects of 9 medium components on the production of antifungal substance by Brevibacillus brevis FM4B.The results showed that glucose,peptone and K2HPO4were the most important medium components.Steepest ascent procedure and response surface methodology based on Box-Behnken experimental design were employed to optimize the three medium components.The optimal fermentation medium for producing antifungal substance was composed of glucose 6.1 g/L,peptone 23.1 g/L,sucrose 31.3 g/L,K2HPO40.825 g/L and MgSO4.7H2O 0.5 g/L.The inhibition spot area of the optimized culture medium after fermentation with Brevibacillus brevis FM4B was increased by 42% compared to that of the original medium.关键词
短短芽孢杆菌/生物防治/Box-Behnken试验/响应曲面法/培养基优化Key words
Brevibacillus brevis/biological control/central composite design/response surface methodology/medium optimization分类
农业科技引用本文复制引用
芮广虎,胡雪芹,殷坤,张洪斌..响应面法优化短短芽孢杆菌FM4B的发酵培养基[J].食品科学,2012,33(15):257-261,5.基金项目
合肥工业大学大学生创新性实验计划项目 （）
