食品科学2012，Vol.33Issue(16)：7-11,5.
油茶皂素大孔树脂纯化工艺优化及LC/TOF-MS分析
Purification by Macroporous Adsorption Resin Chromatography and LC/TOF-MS Analysis of Sasanquasaponin
摘要
Abstract
AB-8 macroporous resin was used to purify commercial crude sasanquasaponin with low purity. The optimal purification process was determined as follows： 30 g/L crude sample solution was loaded followed by washing the resin with 30% ethanol aqueous solution to remove unwanted impurities including most pigments and partial proteins, and the adsorbed sasanquasaponin was finally eluted with 70% ethanol aqueous solution, resulting in a recovery of 55.5% and a purification factor of 1.86. After eighth repeated use, the adsorption and elution capacities of this resin were unchanged basically. Furthermore, the scale-up experiments demonstrated the suitability for industrial production. The LC-TOF-MS analysis indicated that the molecular weight of the purified sasanquasaponin mainly varied between 1170 and 1304 D. In addition, a sasanquasaponin compound with molecular formula C58H89O26 was identified and its structural formula was deduced.关键词
茶皂素/大孔树脂/LC/TOF-MSKey words
sasanquasaponin/macroporous resin/LC/TOF-MS分类
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张新富,朱翔,刘亚军,夏涛,杜先锋,高丽萍,张德龙..油茶皂素大孔树脂纯化工艺优化及LC/TOF-MS分析[J].食品科学,2012,33(16):7-11,5.基金项目
“十一五”国家科技支撑计划项目 （）
