食品科学2012,Vol.33Issue(16):246-250,5.
5株桃、李果实采后褐腐病菌鉴定、rDNA ITS序列与碳源代谢指纹图谱分析
Identification, rDNA ITS Analysis and Carbon Metabolic Fingerprinting of Five Monilinia fructicola Strains Isolated from Postharvest Peach and Plum Fruits
摘要
Abstract
In the present study, four pathogenic strains named as 091,089, 086 and 071, respectively were isolated from infected black amber plum, and one pathogenic strain named as 072 from infected Bayuecui peach during postharvest storage. Morpho- logical observation and rDNA ITS analysis indicated that all five pathogenic strains were Moniliniafructicola. The result obtained also revealed that strains 091 and 089 had a close phylogenetic relationship with Monilinia fructicola (FJ515894). Metabolic fingerprinting suggested that these five strains had the same ability to metabolize 56 carbon sources including 51 optimal ones and 5 unutilized ones. Moniliniafructicola 091 and 089 shared the most similar metabolic fingerprints, whereas Moniliniafructicola 086, 071 and 072 were the most similar. These studies demonstrated that both rDNA ITS analysis and carbon metabolic fingerprinting allows distinguishing among the Moniliniafructicola strains isolated and may be considered as being comparable.关键词
桃果实/李果实/褐腐病菌/rDNA/ITS序列分析/碳源代谢指纹图谱Key words
peach fruit/plum fruit/Moniliniafructicola/rDNA ITS sequence analysis/carbon metabolic fingerprinting分类
生物科学引用本文复制引用
王友升,张燕,陈玉娟..5株桃、李果实采后褐腐病菌鉴定、rDNA ITS序列与碳源代谢指纹图谱分析[J].食品科学,2012,33(16):246-250,5.基金项目
国家“863”计划项目 ()
国家自然科学基金青年科学基金项目 ()
北京市自然科学基金项目 ()