食品科学2012，Vol.33Issue(17)：145-149,5.
滁菊类黄酮3-羟化酶基因的克隆及分子特性
Cloning and Molecular Characterization off3＇h Gene in Dendranthema morifolium
摘要
Abstract
Dendranthema morifolium ＇chuju＇, a traditional Chinese medicine resource unique to Chuzhou, Anhui, was used to clone flavonoid 3 ＇ -hydroxylase （f3 ＇ h） gene with a registration number of HQ256697 in NCBI by homology-based cloning strategy in this study. The results showed that a separate 381 bp DNA fragment was amplified by polymerase chain reaction （PCR） from three ＇chuju＇ samples collected from different places. Sequence analysis showed that the amplified DNA fragments were closely homologous to the 3rd exon off3 ＇h gene in Chrysanthemum morifolium （GU249553） at a homology rate of 98.69% for nucleotide sequences. Five SNPs in the homology area were observed, and only 3 of them resulted in amino acid sequence variations. The homology rates of nucleotide and amino acid sequences between Dendranthema morifolium ＇chuju＇ and Sorghum bicolor, Brassica napus, lpomoea nil Magenta, Malus domestica or lpomoea purpurea were 60%~98% and 68%~97%, respectively. The classification off3＇h gene in different plants was significantly related to their genetic relationship. The f3＇h gene of Dendranthema was more advanced than that ofother plants in system evolution.关键词
滁菊：类黄酮/类黄酮3’．羟化酶基因/外显子3/序列分析Key words
Dendranthema morifolium 'chuju'/flavonoid/f3 'h gene/the 34 exon/sequence analysis分类
生物科学引用本文复制引用
蔡华,许雪峰,诸立新,孙艳辉,代洪亮,崔勇..滁菊类黄酮3-羟化酶基因的克隆及分子特性[J].食品科学,2012,33(17):145-149,5.基金项目
国家自然科学基金项H（30901129 （）
30870359） （）
安徽省高校省级自然科学重点项目 （）
