食品科学2012，Vol.33Issue(17)：184-187,4.
肠出血性大肠杆菌变种的RAPD分型研究
RAPD Typing of Enterohemorrhagic E. coli Mutants
摘要
Abstract
Four enterohemorrhagic Escherichia coli （EHEC） strains were analyzed by random amplified polymorphic DNA （RAPD） method, and the reliability of genotyping of pathogenic E. coli by RAPD was discussed in terms of the major toxic genes, eae and hly and Shiga toxin titer. Two of the test strains carried stx genes but did not express Shiga toxins. Of them, one mutant strain, O157 EC169, had a similar RAPD profile with the other two O157 strains which could express Shiga toxins, while the other mutant, O157 EC130 revealed significant cluster difference from other strains. Several primers with strong repeatability and polymorphism that are available for fast identification of E. coli pathogenic bacteria and the food poisoning traceability were selected from 20 random primers.关键词
肠出血性大肠杆菌（EHEC）/志贺毒素/随机扩增多态性（RAPD）/基因分型Key words
enterohemorrhagic Escherichia coli (EHEC)/Shiga toxins/random amplified polymorphic DNA (RAPD)genotyping分类
医药卫生引用本文复制引用
翟平平,李嘉文,王芳,熊燕,李睿..肠出血性大肠杆菌变种的RAPD分型研究[J].食品科学,2012,33(17):184-187,4.基金项目
湖北省教育厅优秀中青年人才项目 （）
教育部留学回国人员科技启动基金项目 （）
