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脉冲强光对黑曲霉细胞膜损伤及胞内酶活性的影响

张佰清 覃威铭

食品科学2012,Vol.33Issue(17):212-215,4.
食品科学2012,Vol.33Issue(17):212-215,4.

脉冲强光对黑曲霉细胞膜损伤及胞内酶活性的影响

Effect of Intense Pulsed Light Irradiation on Membrane Damage and Intracellular Enzyme Activities in AspergiUus niger

张佰清 1覃威铭1

作者信息

  • 1. 沈阳农业大学食品学院,辽宁沈阳110866
  • 折叠

摘要

Abstract

This study was designed to investigate changes in the membrane permeability and intracellular enzyme activities of Aspergillus niger before and after intense pulsed light irradiation. The results indicated that protein spillage and MDA leakage in Aspergillus niger suspension after intense pulsed light irradiation revealed an obvious increase when compared with the control group. Meanwhile, uneven and wrinkle cell membranes and walls were observed by transmission electron microscopy. Moreover, the activities of dehydrogenase and other major intracellular antioxidant enzymes also revealed a decreasing trend. Therefore, the destruction of the barrier structure of mold could result in changes in the osmotic pressure and cell membrane permeability, thus leading to changes to intracellular substances, and even cell death.

关键词

脉冲强光/黑曲霉/细胞膜损伤/胞内酶活性

Key words

intense pulsed light/Aspergillus niger/membrane damage/intracellular enzyme activity

分类

轻工纺织

引用本文复制引用

张佰清,覃威铭..脉冲强光对黑曲霉细胞膜损伤及胞内酶活性的影响[J].食品科学,2012,33(17):212-215,4.

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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