食品科学2012，Vol.33Issue(18)：83-87,5.
大孔树脂法纯化玉竹总皂苷的工艺研究
Purification of Total Saponins from Rhizoma Polygonati Odorati by Macroporous Resin Adsorption
王晓林 1李珍 1钟方丽1
作者信息
- 1. 吉林化工学院化学与制药工程学院,吉林吉林132022
- 折叠
摘要
Abstract
Objectives: To find out the best type of macroporous resin and determine the optimal process conditions for separation and purification of total saponins from Rhizoma Polygonati Odorati. Methods: The best type of macroporous adsorption resin for total saponins was determined by static adsorption and desorption experiments. Based on one-factor-at-a- time experiments, a four-factor, three-level orthogonal array design was employed for the optimization of four process param- eters for improved adsorption and desorption rates. Results: LSA-33 rnacroporous resin was found to be the best resin among 6 types of macroporous resin tested. The best purification conditions for total saponins were determined as follows: crude extract from Rhizoma Polygonati Odorati (pH 6- 7) was adsorbed for 4 h and then desorbed with 95% ethanol at a solvent-to- solid ratio of 37.5:1 (mL/g) for 2 h. As a result, the content of total saponins in the crude extract was increased to 41.75% from 12.28%. Conclusion: LSA-33 macroporous resin is effective in purifying total saponins from Rhizoma Polygonati Odorati with the advantages of easy operation, high safety and low cost and thus will have high application value.关键词
玉竹/总皂苷/大孔吸附树脂/纯化Key words
Rhizoma Polygonati Odorati total saponins/macroporous resin purification分类
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王晓林,李珍,钟方丽..大孔树脂法纯化玉竹总皂苷的工艺研究[J].食品科学,2012,33(18):83-87,5.
