食品科学2012，Vol.33Issue(18)：123-126,4.
有机相酶法制备溶血磷脂工艺
Enzymatic Hydrolysis of Phospholipids for Preparing Lysophospholipids in Organic Phase
摘要
Abstract
This study deals with the optimization of process conditions for the hydrolysis of phospholipids with phospholipase A1 in hexane for preparing lysophospholipids using response surface methodology. A regression model for acid value of lysophospholipids was established with respect to four hydrolysis parameters. Meanwhile, lysophospholipids were analyzed by infrared spectroscopy and high performance liquid chromatography. The optimal process parameters for preparing lysophospholipids were determined as 50 ℃ of temperature, 13 h of reaction time, 4% of phospholipase Am, 17% of water, and 0.24 g/mL of substrate concentration. The acid value and the contents of phophatidylcholine and 1-acyl-lysophophatidylcholine in the obtained lysophospholipids were 73.8 nag KOH/g, 6.79% and 4.45%, respectively.关键词
溶血磷脂/磷脂/磷脂酶A1/响应面分析法Key words
lysophospholipids/phospholipids/phospholipase A1/response surface methodology (RSM)分类
轻工纺织引用本文复制引用
孙清瑞,张东杰,刘志明,鹿保鑫,王琴,张清荣..有机相酶法制备溶血磷脂工艺[J].食品科学,2012,33(18):123-126,4.基金项目
黑龙江省农垦总局科技攻关项目 （）
