食品科学2012，Vol.33Issue(18)：149-155,7.
RP-HPLC-APCIMS和RP-HPLC-ELSD法分析鸡脂甘油酯组成及结构
Composition and Structural Analysis of Glycerides in Chicken Fat by HPLC-MS and HPLC-ELSD
摘要
Abstract
The composition and structure of glycerides in chicken fat were analyzed by reversed-phase high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC-APCI MS) and reversed-phase high performance liquid chromatography with an evaporative light-scattering detector (RP-HPLC-ELSD). The chromatographic separation was performed on a Cls using a mobile phase of acetonia'ile-methylene chloride (60:40, V/V). Glycerides were monitored by APCI MS in positive mode. According to [M+H]+ and [M+H - RCOOH]+, 26 glycerides were identified. Based on the abundance of [M+H-RCOOH] + and ion fragments from collision induced dissociation (CID) of ion trap, the distribution of fatty acids in glycerides was deduced. Triacylglycerols represented 99.88% of total glycerides as demonstrated through RP-HPLC- ELSD analysis and quantification by peak area normalization. Among these triacylglycerols, 1-palmitoyl-2,3-di-oleoyl-glycerol revealed the highest content (23.65%), followed by 1-palmitoyl-2-oleoyl-3-linoleoyl-glycerol (19.37%), and 1-1inoleoyl-2,3-di- oleoyl-glycerol (16.02%).关键词
鸡脂/甘油酯/高效液相色谱/大气压化学电离质谱/蒸发光散射检测Key words
chicken fat/glyceride/RP-HPLC/APCI MS/ELSD分类
轻工纺织引用本文复制引用
靳林溪,谢建春,王石,孙宝国..RP-HPLC-APCIMS和RP-HPLC-ELSD法分析鸡脂甘油酯组成及结构[J].食品科学,2012,33(18):149-155,7.基金项目
国家自然科学基金面上项目（31171755）~北京市自然科学基金面上项目（2122013）：北京市教委科技发展计划重点项目 （）
“十二五”国家科技支撑计划项目 （）
北京市教委科研基地-科技创新平台.香料化学研究开发平台项目 （）
北京工商大学研究生创新基金项目 （）
