食品科学2012，Vol.33Issue(19)：107-112,6.
嗜热链球菌CH9胞外多糖的分离纯化及结构分析
Purifi cation and Structural Characterization of Exopolysaccharide Produced byStreptococcus thermophilusCH9
摘要
Abstract
Crude exopolysaccharides (EPS) isolated from the fermentation supernatant ofStreptococcus thermophilus CH9 were fractionated by DEAE-52 column chromatography into EPS1, EPS2 and EPS3. Sephadex G-100 column chromatography was used to further fractionate the three factions to harvest their respective major components named as EPS1a, EPS2a and EPS3a, respectively. EPS1a was a neutral polysaccharide with a molecular weight of 1.8× 106 u, and EPS2a and EPS3a were acidic polysaccharides with a molecular weight of 1.06× 106 u and 1.05× 106 u, respectively. Agarose gel electrophoresis analysis indicated that all these three polysaccharides consisted of a single component. UV and IR spectroscopic analyses showed that all of them had characteristic adsorption peaks of polysaccharide. EPS2a and EPS3a might be polysaccharide-protein complexes. Congo red test revealed that EPS1a rather than EPS2a and EPS3a had a three-stranded helix structure. Beta elimination test demonstrated that EPS1a and EPS3a were -O-linked, but EPS2a was a -N-linked polysaccharide.关键词
嗜热链球菌/胞外多糖/分离纯化/结构分析Key words
Streptococcus thermophilusCH9/exopolysaccharides/purifi cation/structural characterization分类
轻工纺织引用本文复制引用
张新源,刘会平,曹春玲,季玲..嗜热链球菌CH9胞外多糖的分离纯化及结构分析[J].食品科学,2012,33(19):107-112,6.基金项目
天津科技大学引进人才科研启动基金项目(20080420) （20080420）
