食品科学2012，Vol.33Issue(19)：124-127,4.
小蓟中甾体类化合物的分离及结构鉴定
Isolation, Purifi cation and Structural Elucidation of Steroids fromCirsium setosum (Willd.) MB
摘要
Abstract
The petroleum ether-soluble fraction of pooled ethanol extracts from the aboveground parts ofCirsium setosum (Willd.) MB was further fractionated by silica gel column chromatography, Sephadex LH-20 column chromatography and preparative HPLC. The resulting sub-fractions were structurally identifi ed by one-dimensional, two-dimensional NMR and MS spectroscopies. Four steroids were obtained, including ergosta-4, 24 (28)-diene-3-one (1), stigmast-4-en-3-one (2), stigmasterol (3) andβ-sitosterol (4). To the best of our knowledge, this is thefi rst report showing the presence of Compounds 1 and 2 in the genusCirsium and the presence of Compounds 1, 2 and 3 inCirsium setosum (Willd.) MB. None of these identifi ed compounds showed cytotoxic activity at a concentration of 1× 10-5 mol/L as determined byin vitro MTT assay.关键词
小蓟/分离纯化/结构鉴定/甾体/细胞毒活性Key words
Cirsium setosum/isolation and purifi cation/structural elucidation/steroids/cytotoxicity分类
生物科学引用本文复制引用
孙珍,李泠鸰,院珍珍,王阿利,李金杰,尚小雅..小蓟中甾体类化合物的分离及结构鉴定[J].食品科学,2012,33(19):124-127,4.基金项目
北京市教育委员会面上项目(KM201111417001) （KM201111417001）
