食品科学2012，Vol.33Issue(19)：211-215,5.
采用全基因组改组技术选育蛋白酶高产菌株
Whole Genome Shuffl ing ofBacillus subtilis for Enhanced Protease Production
摘要
Abstract
Whole genome shuffl ing technique was used to enhance protease production byBacillus subtilis B4. A candidate mutant library was constructed by UV irradiation ofBacillus subtilis B4. Based on the optimum conditions for protoplast preparation and regeneration, multi-parental whole genome shuffl ing was carried out with 4 mutant strains (BRS1, BRS2, BRS5 and BRS6) by PEG mediated protoplasts fusion. Then BRS1, BRS5 and BRS6 were identified by biparental inactivation method as 3 excellent strains with greatly improved protease activity and good genetic stability. The maximum activity was as high as 2175.81 U/mL, which was increased 3.01 fold compared with the initial strain B4.关键词
枯草芽孢杆菌/蛋白酶/基因组改组Key words
Bacillus subtilis/protease/genome shuffl ing分类
生物科学引用本文复制引用
王景会,刘景圣,高岩,马颖辉,李玉秋,牛春华..采用全基因组改组技术选育蛋白酶高产菌株[J].食品科学,2012,33(19):211-215,5.基金项目
国家“863”计划项目(2008AA100802)；吉林省自然科学基金项目(201215196) （2008AA100802）
