食品科学2012，Vol.33Issue(19)：240-243,4.
一株海洋梅久兰链霉菌的分离鉴定及其抗真菌作用研究
Isolation of a MarineStreptomyces mediolani Strain and Its Antimicrobial Activity against Pathogenic Fungi
摘要
Abstract
A total of 22 strains of marine actinomycetes were isolated from seawater, sea mud, offshore soils, marine animals and marine plant samples collected from the sea area near Lianyungang by gradient dilution method. The antimicrobial activity of these strain isolates was tested by plate confrontation method. It was found that 9 of them had antimicrobial activity. Strain ZW-1 had the strongest antimicrobial activity, and it and its fermentation broth possessed strong inhibitory effects on 8 plant pathogenic fungi includingFusarium nivale, Fusarium graminearum,Alternaria alternate, Sclerotinia sclerotiorum,Alternaria solani,Helminthosporium carbonum,Fusarium oxysporum f.sp.niveum, andFusarium oxysporum f.sp.vasinfectum. In addition to causing cell wall expansion and protoplast contraction in pathogenic fungal mycelia, this strain and its fermentation broth could inhibit the germination ofA. solani conidiophores and the elongation of germ tubes. Based on its culture characteristics, morphology, physiological and biochemical properties, and 16S rDNA sequence, ZW-1 strain was identifi ed asStreptomyces mediolani.关键词
海洋链霉菌ZW-1菌株/抑制作用/分离鉴定Key words
marineStreptomyces mediolaniZW-1/antimicrobial activity/isolation and identifi cation分类
生物科学引用本文复制引用
暴增海,马桂珍,王淑芳,葛平华,吴少杰,付泓润..一株海洋梅久兰链霉菌的分离鉴定及其抗真菌作用研究[J].食品科学,2012,33(19):240-243,4.基金项目
江苏省“六大人才高峰”资助项目；中央财政支持地方高校发展专项资金科研创新团队项目 （）
