食品科学2012，Vol.33Issue(19)：310-314,5.
灵芝多糖联合5-FU对人结肠癌HCT-116细胞增殖及凋亡的影响
Effect of CombinedGanoderma lucidum Polysacchrides (GLP) and Fluorouracil on Proliferation and Apoptosis in Human Colon Carcinoma HCT-116 Cells
梁曾恩妮 1易有金 2郭雨桐 1王仁才 1熊兴耀1
作者信息
- 1. 湖南农业大学园艺园林学院，湖南长沙 410128
- 2. 湖南农业大学食品科技学院，湖南长沙 410128
- 折叠
摘要
Abstract
Objective: To explore the effect ofGanoderma lucidum polysacchrides (GLP) combined with fluorouracil treatment on the proliferation and apoptosis of human colon carcinoma HCT-116 cellsin vitro. Methods: The inhibitory effects of both drugs on the proliferation of HCT-116 cells were measured by using MTT assay. The combinatorial index method was used to analyze their synergistic inhibitory effect. Observations of morphological changes were carried out with Hoechst33258fl uorescence staining. The impact of GLP on cell cycle and apoptosis rate of HCT-116 cells was evaluated byfl ow cytometry (FCM). Results: GLP and 5-FU treatment alone suppressed the proliferation of HCT-116 cells. GLP treatment (>659μg/mL) in combination with 5-FU (>1.6μg/mL) revealed an obvious synergistic effect on the proliferation of HCT-116 cells (CI<1) which exhibited a signifi cant difference when compared with 5-FU treatment alone (P<0.01) in a time-dependent manner. After treatments for 48 h, the cells showed typical apoptosis characteristics. The apoptotic HCT-116 cells were 36.14% after GLP (5 mg/mL) combined with 5-FU (50μg/mL) treatment for 24 h, which was higher than that obtained from 5-FU treatment alone (26.07%). The apoptotic HCT-116 cells induced by 1.25 mg/mL GLP combined with 12.5μg/mL 5-FU, 2.5 mg/mL GLP combined with 25μg/mL 5-FU, and 5 mg/mL GLP combined with 50μg/mL 5-FU for 48 h were 20.95%, 32.87% and 30.01%, respectively, showing an increase compared with 5-FU (50μg/mL) treatment alone (14.51%). The arrested cel cycle in combinatorial treatment group was observed at S phase. Conclusion: GLP at higher concentration combined with 5-FU can synergisticaly suppress the proliferation of human colon cancer HCT-116 cels, and enhanced apoptosis and cel cycle arrest in HCT-116 cels.关键词
灵芝多糖/结肠癌/5-FU/凋亡/细胞周期Key words
Ganoderma lucidum polysacchrides/colon carcinoma/fl uorouracil/apoptosis/cell cycle分类
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梁曾恩妮,易有金,郭雨桐,王仁才,熊兴耀..灵芝多糖联合5-FU对人结肠癌HCT-116细胞增殖及凋亡的影响[J].食品科学,2012,33(19):310-314,5.
